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KMID : 0385219940040010001
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Korean Journal of Gerontology
1994 Volume.4 No. 1 p.1 ~ p.7
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Studies on Responsible Factor for Microsomal Membrane Rigidification by Liposomal Manuplations
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Choe Myeon
Yu Byung-Pal
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Abstract
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To test hypothesis that age-related lipid peroxidation might play a greater role than cholesterol in causing rigidity in senescent membranes, we conducted a series of experiments to manipulate membrane fluidity by comparing the effects of peroxidized and cholesterol-load liposomes. Small unilamella. liposomes were prepared and either peroxidized by Fe++ - -ADP-ascorbic acid or loaded with cholesterol. After incorporation of the liposomes into rat liver microsome, membrane fluidity was quantitated by measuring changes in polarization and expressed as l/polarization. Because differential sensitivity of membranes to peroxidation and cholesterol increase occurred, incorporation of peroxidized liposomes (peroxidation) appeared to be a more effective factor in inducing membrane rigidity than cholesterol increase. Membrane rigidity can be readily influenced by lipid peroxidation of fatty acids in membrane phospholipids, irrespective of influence by cholesterol. Therefore, these results support the proposal that lipid peroxidation is more potent than increased cholesterol in membrane rigidification.
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KEYWORD
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microsomal membrane rigidity, peroxidized liposomes, cholesterol-load liposomes
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